{"id":302624,"date":"2024-10-19T20:40:38","date_gmt":"2024-10-19T20:40:38","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-208142019\/"},"modified":"2024-10-25T18:12:28","modified_gmt":"2024-10-25T18:12:28","slug":"bs-iso-208142019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-208142019\/","title":{"rendered":"BS ISO 20814:2019"},"content":{"rendered":"
This document specifies a method for the measurement of the photocatalytic activity (PCA) of nanoparticles (NPs), suspended in an aqueous environment in physiologically relevant conditions, by measuring the ultraviolet (UV)-induced nicotine adenine dinucleotide hydrate (NADH) oxidation.<\/p>\n
The measurement is intended to assess the potential for the photo-toxicity of nanomaterials. The method is also applicable to NP aggregates and agglomerates.<\/p>\n
PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
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2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
6<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
8<\/td>\n | 1 Scope 2 Normative references 3 Terms, definitions, symbols and abbreviated terms 3.1 Terms and definitions <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 3.2 Symbols and abbreviated terms <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 4 Description of the test method 5 Reagents and apparatus 5.1 Reagents <\/td>\n<\/tr>\n | ||||||
11<\/td>\n | 5.2 Apparatus 6 Measurement procedure 6.1 Measurement of NP suspension basic properties 6.1.1 UV-Vis absorption spectrum measurement <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 6.1.2 NP suspension stability measurement 6.2 UV trans-illuminator light intensity calibration based on 2NB actinometry <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 6.3 Measurement of NADH solution fluorescence intensity 6.3.1 NADH photo-oxidation rate measurement at various NP concentrations <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 6.3.2 Calculation of NADH photo-oxidation rate at various NP concentrations <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | 7 Test report 7.1 Information <\/td>\n<\/tr>\n | ||||||
16<\/td>\n | 7.2 Report data format 7.2.1 Correction factors C(i,j) obtained by actinometry (see 7.2) with \u03bb(max,TI) 7.2.2 Calibrated slope of NADH fluorescence decrease <\/td>\n<\/tr>\n | ||||||
17<\/td>\n | 7.2.3 Plot of kapp versus NP concentration 7.2.4 NADH equivalent specific PCA 8 Precision 8.1 Repeatability 8.2 Reproducibility <\/td>\n<\/tr>\n | ||||||
18<\/td>\n | Annex A (normative) Schematic diagram of 96-well positioning block <\/td>\n<\/tr>\n | ||||||
19<\/td>\n | Annex B (informative) Sample calibration of UV trans-illuminator light intensity <\/td>\n<\/tr>\n | ||||||
24<\/td>\n | Annex C (informative) Interlaboratory comparison study of TiO2 NP PCA <\/td>\n<\/tr>\n | ||||||
29<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Nanotechnologies. Testing the photocatalytic activity of nanoparticles for NADH oxidation<\/b><\/p>\n |