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ASTM-E1759:2003 Edition

ASTM-E1759:2003 Edition

$35.75

E1759-95(2003) Standard Test Method for Isoaspartic Acid in Proteins: Method for the Determination of Asparagine Deamidation Products

Published By Publication Date Number of Pages
ASTM 2003 4
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ASTM E1759-95-Reapproved2003

Withdrawn Standard: Standard Test Method for Isoaspartic Acid in Proteins: Method for the Determination of Asparagine Deamidation Products (Withdrawn 2003)

ASTM E1759

Scope

1.1 This test method covers the determination of isoaspartic acid residues in a protein or peptide sample. This test method is applicable for the determination of isoaspartic acid residues in a sample in the range of 2.5-50 μmol/L. Higher concentrations can be determined following dilution. The reported lower range is based on single-operator precision.

1.2 The values stated in SI units are to be regarded as the standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

deamidation; isoaspartic acid residue; protein damage

ICS Code

ICS Number Code 07.030 (Physics. Chemistry)

DOI: 10.1520/E1759-95R03

PDF Catalog

PDF Pages PDF Title
1 Scope
Terminology
Summary of Test Method
Significance and Use
2 Interfering Substances
Apparatus
Reagents and Materials
Calibration
Procedure
3 Calculation
Precision and Bias
Keywords
X1. ADDITIONAL TECHNICAL CONSIDERATIONS
X1.1 Testing Protein Samples in Complex Aqueous Solutions
X1.2 Confirmation of the Accessibility of Isoaspartic Acid Residues for a New Protein
4 X1.3 Confirmation of the Useful Range of the Assay for a New Protein

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