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BS EN ISO 11290-2:2017

BS EN ISO 11290-2:2017

$189.07

Microbiology of the food chain. Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Enumeration method

Published By Publication Date Number of Pages
BSI 2017 38
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This document specifies a horizontal method for

  • the enumeration of L. monocytogenes, and

  • the enumeration of Listeria spp. (including L. monocytogenes).

This document is applicable to

  • products intended for human consumption and for the feeding of animals, and

  • environmental samples in the area of food production and food handling.

It is possible that certain additionally described Listeria species may not be enumerated or confirmed by this method.[3],[6],[9],[11]

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
10 3 Terms and definitions
4 Principle
4.1 General
4.2 Initial suspension
4.3 Surface plating
4.4 Incubation
11 4.5 Confirmation
4.6 Enumeration
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
12 8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.3 Enumeration of characteristic colonies
13 9.4 Confirmation of L. monocytogenes or Listeria spp.
9.4.1 Selection of colonies for confirmation
14 9.4.2 Confirmation of L. monocytogenes
17 9.4.3 Confirmation of Listeria spp.
18 9.5 Interpretation of morphological and physiological properties and of the biochemical reactions
9.6 Additional characterization of isolated strains (optional)
10 Expression of results
11 Performance characteristics of the method
11.1 Method validation study
19 11.2 Repeatability limit
11.3 Reproducibility limit
12 Test report
20 13 Quality assurance
21 Annex A (normative) Diagram of procedure
22 Annex B (normative) Composition and preparation of media and reagents
30 Annex C (informative) Distinction of Listeria spp. from other genera
31 Annex D (informative) Reactions for the identification of Listeria species
33 Annex E (informative) Results of interlaboratory studies for enumeration of Listeria monocytogenes
36 Bibliography

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BS EN ISO 11290-2:2017
$189.07