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BS EN ISO 21528-1:2017

BS EN ISO 21528-1:2017

$142.49

Microbiology of the food chain. Horizontal method for the detection and enumeration of Enterobacteriaceae – Detection of Enterobacteriaceae

Published By Publication Date Number of Pages
BSI 2017 28
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This document specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to

  • products intended for human consumption and the feeding of animals, and

  • environmental samples in the area of primary production, food production and food handling.

This method is applicable

  • when the microorganisms sought are expected to need resuscitation by enrichment, and

  • when the number sought is expected to be below 100 per millilitre or per gram of test sample.

A limitation on the applicability of this document is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).

NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
4.1 Enrichment in non-selective medium
4.2 Isolation and selection for confirmation
4.3 Confirmation
5 Diluent, culture media and reagent
6 Equipment and consumables
11 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
12 9.2 Test portion and initial suspension
9.3 Enrichment
9.4 Isolation and selection for confirmation
9.4.1 Isolation
9.4.2 Selection of colonies for confirmation
9.5 Subculturing selected colonies
9.6 Biochemical confirmation tests
9.6.1 Oxidase reaction
13 9.6.2 Fermentation test
10 Expression of results
11 Precision
11.1 Interlaboratory study
11.2 Sensitivity
11.3 Specificity
12 Test report
14 13 Quality assurance
15 Annex A (informative) Enumeration by MPN technique
18 Annex B (normative) Culture media and reagents
23 Annex C (informative) Method validation studies and performance characteristics
25 Bibliography

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BS EN ISO 21528-1:2017
$142.49