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BSI PD CEN/TS 17713:2022

BSI PD CEN/TS 17713:2022

$102.76

Plant biostimulants. Determination of Azospirillum spp.

Published By Publication Date Number of Pages
BSI 2022 18
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This document provides the methodology for the enumeration and determination of Azospirillum spp. in plant biostimulant products in accordance to the Regulation of EU fertilising products [1].

PDF Catalog

PDF Pages PDF Title
2 National foreword
5 European foreword
6 Introduction
7 1 Scope
2 Normative references
3 Terms and definitions
4 Enumeration of Azospirillum spp.
4.1 General
4.2 Sampling
4.3 Preparation of the sample
4.3.1 Sample preparation
8 4.3.2 Liquid -based water- formulations
4.3.3 Liquid – oil based emulsifiable concentrate (EC) formulations
4.3.4 Solid – Wettable Powder (WP) formulations
4.3.5 Solid – Water dispersible granules (WDG) formulations
4.3.6 Solid – Pellets, granules, microgranules (slow release) formulations
4.3.7 Solid – substrate
4.4 Requirements (apparatus)
9 4.5 Preparation of the culture media
4.6 Preparation of serial dilution for MPN count
4.7 Incubation of tubes
4.8 Counting
4.9 Method for estimating MPN count
10 5 Species determination of Azospirillum spp. via genetic analysis
5.1 General
5.2 Preparation of the sample for the genomic DNA extraction
5.2.1 Isolation and preparation of the microorganism
5.2.2 Sample concentration
5.2.3 DNA extraction and storage
5.2.4 Partial PCR amplification of the 16S rRNA genes
12 Annex A (normative) Formula of the culture media
14 Annex B (normative) Table of MPN values
16 Bibliography

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BSI PD CEN/TS 17713:2022
$102.76