{"id":338136,"date":"2024-10-19T23:39:19","date_gmt":"2024-10-19T23:39:19","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-166182015\/"},"modified":"2024-10-25T22:45:12","modified_gmt":"2024-10-25T22:45:12","slug":"bs-en-166182015","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-166182015\/","title":{"rendered":"BS EN 16618:2015"},"content":{"rendered":"

This European Standard specifies a method for the determination of acrylamide in bakery ware such as bread, toasted bread, crisp bread, butter cookies, and biscuits, as well as potato products such as potato chips, potato crisps, and potato pan cake and roasted coffee, by liquid chromatography in combination with electrospray ionization and tandem mass spectrometry (LC-ESI-MS\/MS). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples, ranging from 14,3 \u00b5g\/kg to 9 083 \u00b5g\/kg. It was developed at the Swedish National Food Administration and validated in a study organized by the Directorate General Joint Research Centre (DG JRC), Swedish National Food Administration and the Nordic Committee on Food Analysis (NMKL), see [1]<\/span> and [2]<\/span>.<\/p>\n

The limit of quantification (LOQ) depends on the type of instrument used and on the actual performance of the instrument. The majority of the laboratories participating in the validation study were able to determine acrylamide in a butter cookie sample at a level of 14,3 \u00b5g\/kg. Thus, the validation by interlaboratory study showed that LOQ can be expected to be in the range between below 15 \u00b5g\/kg and 30 \u00b5g\/kg.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
5<\/td>\nForeword <\/td>\n<\/tr>\n
6<\/td>\n1 Scope
2 Normative references
3 Principle
4 Reagents <\/td>\n<\/tr>\n
7<\/td>\nFigure 1 \u2014 Acrylamide
Figure 2 \u2014 Deuterium-labelled acrylamide <\/td>\n<\/tr>\n
8<\/td>\nTable 1 \u2014 Preparation of LC-MS calibration solutions <\/td>\n<\/tr>\n
9<\/td>\nTable 2 \u2014 Relation between acrylamide contents of calibration solutions and contents in food
5 Apparatus <\/td>\n<\/tr>\n
10<\/td>\n6 Sample preparation
6.1 General <\/td>\n<\/tr>\n
11<\/td>\n6.2 Extraction
6.2.1 Extraction procedure for bakery ware and potato product samples
6.2.2 Extraction procedure for coffee samples
6.3 Cleanup
6.3.1 Cleanup for bakery and potato product sample <\/td>\n<\/tr>\n
12<\/td>\n6.3.2 Cleanup for coffee samples
7 Measurement
7.1 LC-MS\/MS determination
7.1.1 LC-MS\/MS conditions <\/td>\n<\/tr>\n
13<\/td>\nTable 3 \u2014 LC-MS\/MS conditions <\/td>\n<\/tr>\n
14<\/td>\n7.1.2 System suitability
7.1.3 Regeneration of HPLC column
7.2 Batch (sequence) composition <\/td>\n<\/tr>\n
15<\/td>\n7.3 Identification and calculation of results
7.4 Recovery
8 Determination of concentrations <\/td>\n<\/tr>\n
17<\/td>\n9 Precision
9.1 General
9.2 Repeatability <\/td>\n<\/tr>\n
18<\/td>\n9.3 Reproducibility <\/td>\n<\/tr>\n
19<\/td>\n10 Test report <\/td>\n<\/tr>\n
20<\/td>\nAnnex A (informative) Typical chromatograms
Figure A.1 \u2014 Typical chromatogram of a potato chips sample containing acrylamide <\/td>\n<\/tr>\n
22<\/td>\nAnnex B (informative) Precision data
Table B.1 \u2014 Precision data for acrylamide in butter cookies, toasted bread and spiced biscuits <\/td>\n<\/tr>\n
23<\/td>\nTable B.2 \u2014 Precision data for acrylamide in potato crisps, mashed potato powder and crisp bread <\/td>\n<\/tr>\n
24<\/td>\nTable B.3 \u2014 Precision data for acrylamide in potato crisps <\/td>\n<\/tr>\n
25<\/td>\nTable B.4 \u2014 Precision data for acrylamide in roasted coffee <\/td>\n<\/tr>\n
26<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Food analysis. Determination of acrylamide in food by liquid chromatography tandem mass spectrometry (LC-ESI-MS\/MS)<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2015<\/td>\n28<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338142,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338136","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338136","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338142"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338136"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338136"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}