This document gives general guidelines for enumeration and detection of aerobic mesophilic bacteria present in cosmetics<\/p>\n
by counting the colonies on agar medium after aerobic incubation, or<\/p>\n<\/li>\n
by checking the absence of bacterial growth after enrichment.<\/p>\n<\/li>\n<\/ul>\n
Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.<\/p>\n
If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.<\/p>\n
In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity, hydro-alcoholic products, extreme pH values, etc.<\/p>\n
| PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
|---|---|---|---|---|---|---|---|
| 2<\/td>\n | National foreword <\/td>\n<\/tr>\n | ||||||
| 4<\/td>\n | European foreword Endorsement notice <\/td>\n<\/tr>\n | ||||||
| 7<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
| 9<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions <\/td>\n<\/tr>\n | ||||||
| 10<\/td>\n | 4 Principle 4.1 General 4.2 Plate count 4.3 Membrane filtration <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | 4.4 Detection of bacteria by enrichment 5 Diluents, neutralizers and culture media 5.1 General 5.2 Neutralizing diluents and diluents <\/td>\n<\/tr>\n | ||||||
| 12<\/td>\n | 5.3 Diluent for the bacterial suspension (tryptone sodium chloride solution) 5.4 Culture media <\/td>\n<\/tr>\n | ||||||
| 15<\/td>\n | 6 Apparatus and glassware 7 Strains of microorganisms 8 Handling of cosmetic products and laboratory samples 9 Procedure 9.1 General recommendation 9.2 Preparation of the initial suspension 9.2.1 General <\/td>\n<\/tr>\n | ||||||
| 16<\/td>\n | 9.2.2 Water-miscible products 9.2.3 Water-immiscible products 9.3 Counting methods 9.3.1 Dilutions for counting methods 9.3.2 Plate-count methods <\/td>\n<\/tr>\n | ||||||
| 17<\/td>\n | 9.4 Enrichment 9.4.1 General 9.4.2 Incubation of the sample 10 Counting of colonies (plate counts and membrane filtration methods) 11 Detection of growth (enrichment method) <\/td>\n<\/tr>\n | ||||||
| 18<\/td>\n | 12 Expression of results 12.1 Method of calculation for plate count <\/td>\n<\/tr>\n | ||||||
| 19<\/td>\n | 12.2 Interpretation 12.3 Examples <\/td>\n<\/tr>\n | ||||||
| 21<\/td>\n | 12.4 Detection after enrichment 13 Neutralization of the antimicrobial properties of the product 13.1 General <\/td>\n<\/tr>\n | ||||||
| 22<\/td>\n | 13.2 Preparation of inoculum 13.3 Suitability of counting methods 13.3.1 Principle 13.3.2 Suitability test of the pour-plate method 13.3.3 Suitability of the surface spread method 13.3.4 Suitability of the membrane filtration method <\/td>\n<\/tr>\n | ||||||
| 23<\/td>\n | 13.4 Suitability of the detection method by enrichment 13.4.1 Procedure 13.4.2 Interpretation of results 13.5 Interpretation of suitability test results <\/td>\n<\/tr>\n | ||||||
| 24<\/td>\n | 14 Test report <\/td>\n<\/tr>\n | ||||||
| 25<\/td>\n | Annex\u00a0A (informative) Other neutralizing diluents <\/td>\n<\/tr>\n | ||||||
| 27<\/td>\n | Annex\u00a0B (informative) Other diluents <\/td>\n<\/tr>\n | ||||||
| 28<\/td>\n | Annex\u00a0C (informative) Other culture media <\/td>\n<\/tr>\n | ||||||
| 31<\/td>\n | Annex\u00a0D (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids <\/td>\n<\/tr>\n | ||||||
| 32<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Cosmetics. Microbiology. Enumeration and detection of aerobic mesophilic bacteria<\/b><\/p>\n |