This document specifies general rules for the enumeration of lactobacilli in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain lactobacilli as a single microorganism component or in a mixture with other microorganisms. Applying the method to premixtures and compound feeds with critical amounts of copper demands a special procedure (see A.2). The document is not applicable to mineral feeds, which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) No 767\/2009) [3]. There are different categories of feed samples: a) Additives containing about 1010 colony forming units (CFU)\/g; b) Premixtures containing about 1011 CFU\/kg; c) Compound feeds, meal or pellets which contain about 109 CFU\/kg.<\/p>\n
| PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
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| 1<\/td>\n | 30462974 <\/td>\n<\/tr>\n | ||||||
| 31<\/td>\n | A-30372726 <\/td>\n<\/tr>\n | ||||||
| 32<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
| 38<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions 4 Principle <\/td>\n<\/tr>\n | ||||||
| 39<\/td>\n | 5 Diluents and culture media 5.1 Diluents 5.1.1 Diluent for initial suspension <\/td>\n<\/tr>\n | ||||||
| 40<\/td>\n | 5.1.2 Diluents for serial dilutions 5.2 Culture media 5.2.1 General 5.2.2 Composition <\/td>\n<\/tr>\n | ||||||
| 41<\/td>\n | 5.2.3 Preparation <\/td>\n<\/tr>\n | ||||||
| 42<\/td>\n | 6 Apparatus <\/td>\n<\/tr>\n | ||||||
| 43<\/td>\n | 7 Sampling 8 Preparation of test sample 9 Procedure 9.1 Samples with a single or multi-component added microflora 9.2 Preparation of poured agar plates for spread plate method <\/td>\n<\/tr>\n | ||||||
| 44<\/td>\n | 9.3 Preparation of MRS agar for pour plate method 9.4 Preparation of the initial suspension and decimal dilutions <\/td>\n<\/tr>\n | ||||||
| 45<\/td>\n | 9.5 Inoculation and incubation of plates 9.5.1 General 9.5.2 Spread plate method <\/td>\n<\/tr>\n | ||||||
| 46<\/td>\n | 9.5.3 Pour plate method 9.6 Enumeration of colonies 9.7 Confirmation <\/td>\n<\/tr>\n | ||||||
| 47<\/td>\n | 10 Expression of results 11 Precision 11.1 General 11.2 Interlaboratory study 11.3 Repeatability <\/td>\n<\/tr>\n | ||||||
| 48<\/td>\n | 11.4 Reproducibility 12 Test report <\/td>\n<\/tr>\n | ||||||
| 49<\/td>\n | Annex A (informative)Notes on the procedure A.1 General A.2 Critical copper concentration <\/td>\n<\/tr>\n | ||||||
| 51<\/td>\n | Annex B (informative)Results of the interlaboratory study B.1 General B.2 Results of the interlaboratory study <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Tracked Changes. Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Lactobacillus spp. used as feed additive<\/b><\/p>\n |