API TR 410-1996

$29.25

Chromosome Aberrations in Chinese Hamster Ovary (CHO) Cells Exposed to Tertiary Amyl Methyl Ether (TAME)

Published By	Publication Date	Number of Pages
API	1996	57

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Description

The test article, Tertiary Amyl Methyl Ether (TAME), was tested in the Chromosome Aberration Assay using Chinese hamster ovary (CHO) cells, in both the absence and presence of Aroclor-induced rat liver S9 (fraction of extracted biotransformation enzymes capable of metabolizing putative promutagens into the ultimate mutagenic agent) at doses of 313 to 5000 pg/ml. All concentrations tested in both the non-activated and the S9-activated test systems were soluble in treatment medium. Toxicity, i.e., cell growth inhibition, was 43°/0 at the 5000 pg/ml dose level in the non-activated test system and 72% at the 5000 ~g/ml dose level in the S9-activated test system. No positive response was seen in the non-activated test system. In the S9-activated test system there was a positive response with an increasing concentration of the test article. Under the conditions of this study, the test article, Tertiary Amyl Methyl Ether (TAME), was concluded to be positive in the Chromosome Aberration Assay using CHO cells.

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