ASTM-D4454 2002
$35.75
D4454-85(2002) Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy
| Published By | Publication Date | Number of Pages |
| ASTM | 2002 | 3 |
1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to environmental waters and potable waters.
1.2 Certain types of debris and other microorganisms may fluoresce in acridine-orange stained smears.
1.3 The procedure described requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.
1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.
1.5 Approximately 10 4 cells/mL are required for detection by this test method.
1.6 Minimal cell size which allows the detection of formazan deposits is represented by bacteria of 0.4 m. 2
1.7 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 1 | Scope Referenced Documents Terminology Summary of Test Method Significance and Use Apparatus |
| 2 | Reagents and Materials Procedure Enumeration and Density Calculation Report |
| 3 | Precision and Bias TABLE 1 |
