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BSI PD CEN/TS 17701:2022

BSI PD CEN/TS 17701:2022

$102.76

Plant biostimulants. Determination of the yeast and mould content

Published By Publication Date Number of Pages
BSI 2022 20
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This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulants intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C ± 2,5 °C. This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilisers Regulation [1]. If necessary, yeast and mould enumerated can be identified using suitable identification tests.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
3 Terms and definitions
9 4 Principles
4.1 General
4.2 Plate count method
5 Diluent and culture media
5.1 General
5.2 Diluent
5.3 Culture media
6 Apparatus and glassware
10 7 Handling of plant biostimulants and sampling
8 Procedure
8.1 General
8.2 Test portion and initial suspension
8.2.1 General
8.2.2 Liquid – water based – formulations
8.2.3 Liquid – oil based (emulsifiable concentrate – EC) formulations
8.2.4 Solid – Wettable Powder (WP) formulations
8.2.5 Solid – Water dispersible granules (WDG) formulations
11 8.2.6 Solid – Pellets, granules, microgranules (slow release) formulations
8.2.7 Solid – substrate
8.3 Serial dilutions
8.4 Plate-count methods
8.4.1 Pour-plate method
8.4.2 Surface spread method
8.4.3 Incubation
12 9 Counting of colonies
10 Expression of results
10.1 Method of calculation
13 10.2 Interpretation
14 11 Test report
15 Annex A (informative)Diluent
A.1 General
A.2 Phosphate Buffered Saline (PBS)
A.2.1 Composition
A.2.2 Preparation
16 Annex B (informative)Culture media
B.1 General
B.2 Sabouraud dextrose chloramphenicol agar medium (SDCA)
B.2.1 Composition
B.2.2 Preparation
17 B.3 Potato dextrose agar (PDA) medium with antibiotics
B.3.1 Composition
B.3.2 Preparation
B.4 Glucose -peptone (GP) agar medium with antibiotics
B.4.1 Composition
B.4.2 Preparation
18 B.5 Malt extract agar (MEA) medium with antibiotics
B.5.1 Composition
B.5.2 Preparation

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BSI PD CEN/TS 17701:2022
$102.76