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BSI PD CEN/TS 17709:2022

BSI PD CEN/TS 17709:2022

$102.76

Plant biostimulants. Determination of Azotobacter spp.

Published By Publication Date Number of Pages
BSI 2022 16
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This document was developed to provide the methodology for the enumeration and determination of Azotobacter sp. in plant biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

PDF Catalog

PDF Pages PDF Title
2 National foreword
5 European foreword
6 Introduction
7 1 Scope
2 Normative references
3 Terms and definitions
4 Enumeration of Azotobacter spp.
4.1 General
4.2 Sample preparation
4.2.1 General
4.2.2 Liquid (based water) formulations
4.2.3 Liquid – based oil, emulsifiable concentrate (EC) formulations
4.2.4 Solid – Wettable Powder (WP) formulations
8 4.2.5 Solid – Water dispersible granules (WDG) formulations
4.2.6 Solid – Pellets, granules, microgranules (slow release) formulations
4.2.7 Solid – substrate
4.3 Serial dilution
9 4.4 Plate counts of Azotobacter sp in sterile diluent
4.5 Spread-plate counting with ASHBY SUCROSE AGAR [6]
10 4.6 Calculation
5 Species determination of Azotobacter sp. via genetic analysis
5.1 General
5.2 Preparation of the sample for the genomic DNA extraction
5.2.1 Isolation and preparation of the microorganism
5.2.2 Sample concentration
5.2.3 DNA extraction and storage
11 5.2.4 Partial PCR amplification of the 16S rRNA Genes
13 Annex A (informative) Formula of culture media
15 Bibliography

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BSI PD CEN/TS 17709:2022
$102.76