{"id":338087,"date":"2024-10-19T23:39:03","date_gmt":"2024-10-19T23:39:03","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-12823-12014\/"},"modified":"2024-10-25T22:44:52","modified_gmt":"2024-10-25T22:44:52","slug":"bs-en-12823-12014","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-12823-12014\/","title":{"rendered":"BS EN 12823-1:2014"},"content":{"rendered":"

This European Standard specifies a method for the determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC). This method has been validated in an interlaboratory study with samples of margarine and milk powder with all-E-retinol levels ranging from 653 \u00b5g\/100 g to 729 \u00b5g\/100 g and with 13-Z-retinol levels ranging from 30 \u00b5g\/100 g to 39 \u00b5g\/100 g. The determination of vitamin A content is carried out by the measurement of all-E-retinol, 13-Z-retinol and \u03b2-carotene. This part covers the measurement of all-E-retinol and 13-Z-retinol.<\/p>\n

The extract obtained after saponification in this method can be used for the determination of \u03b2-carotene, as described in EN 12823-2:2000, Foodstuffs – Determination of vitamin A by high performance liquid chromatography – Part 2: Measurements of \u03b2-carotene<\/i>. In this case, the saponification temperature should preferably not exceed 80 \u00b0C in order to prevent isomerisation and oxidation of \u03b2-carotene.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
4<\/td>\nContents Page <\/td>\n<\/tr>\n
5<\/td>\nForeword <\/td>\n<\/tr>\n
6<\/td>\n1 Scope
2 Normative references
3 Principle
4 Reagents <\/td>\n<\/tr>\n
9<\/td>\n5 Apparatus <\/td>\n<\/tr>\n
10<\/td>\n6 Procedure
6.1 General
6.2 Preparation of the test sample
6.3 Preparation of the sample test solution
6.3.1 Saponification
Table 1 \u2014 Examples of suitable ratios of reagents <\/td>\n<\/tr>\n
11<\/td>\n6.3.2 Extraction
6.3.3 Evaporation
6.3.4 Dilution
6.4 Preparation of the calibration solutions
6.5 Identification and purity check <\/td>\n<\/tr>\n
12<\/td>\n6.6 Determination
7 Calculation <\/td>\n<\/tr>\n
13<\/td>\n8 Precision
8.1 General
8.2 Repeatability <\/td>\n<\/tr>\n
14<\/td>\n8.3 Reproducibility
9 Test report <\/td>\n<\/tr>\n
15<\/td>\nAnnex A (informative) Examples of HPLC chromatograms
Figure A.1 \u2014 HPLC chromatograms of vitamin A standards (a), vitamin A in margarine (b) and milk powder (c) <\/td>\n<\/tr>\n
16<\/td>\nAnnex B (informative) Precision data
Table B.1 \u2014 Precision data for margarine and milk powder <\/td>\n<\/tr>\n
17<\/td>\nAnnex C (informative) Alternative HPLC systems
Table C.1 \u2014 Alternative HPLC systems and chromatographic conditions <\/td>\n<\/tr>\n
18<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Determination of vitamin A by high performance liquid chromatography – Measurement of all-E-retinol and 13-Z-retinol<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2014<\/td>\n20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338094,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338087","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338087","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338094"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338087"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338087"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338087"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}