{"id":338156,"date":"2024-10-19T23:39:25","date_gmt":"2024-10-19T23:39:25","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-161872015\/"},"modified":"2024-10-25T22:45:21","modified_gmt":"2024-10-25T22:45:21","slug":"bs-en-161872015","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-161872015\/","title":{"rendered":"BS EN 16187:2015"},"content":{"rendered":"

This European Standard specifies a method for the determination of fumonisin B 1<\/sub> (FB 1<\/sub>) and fumonisin B 2<\/sub> (FB 2<\/sub>) in processed maize-containing foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection (FLD). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 112 \u00b5g\/kg to 458 \u00b5g\/kg for FB 1<\/sub>+FB 2<\/sub>, 89 \u00b5g\/kg to 384 \u00b5g\/kg for FB 1<\/sub> and 22 \u00b5g\/kg to 74 \u00b5g\/kg for FB 2<\/sub>.<\/p>\n

For further information on the validation, see Clause 8<\/span> and Annex B<\/span>.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
5<\/td>\nForeword <\/td>\n<\/tr>\n
6<\/td>\n1 Scope
2 Normative references
3 Principle
4 Reagents <\/td>\n<\/tr>\n
8<\/td>\nTable 1 \u2014 Preparation of mixed FBs calibration solutions for HPLC <\/td>\n<\/tr>\n
9<\/td>\n5 Apparatus <\/td>\n<\/tr>\n
10<\/td>\nTable 2 \u2014 Gradient conditions
6 Procedure
6.1 Extraction <\/td>\n<\/tr>\n
11<\/td>\n6.2 Immunoaffinity column cleanup
6.3 Spiking procedure
6.4 Derivatisation and HPLC determination
6.4.1 Automated pre-column derivatisation programme <\/td>\n<\/tr>\n
12<\/td>\n6.4.2 HPLC injections
6.4.3 Peak identification
6.4.4 Determination
7 Calculation <\/td>\n<\/tr>\n
13<\/td>\n8 Precision
8.1 General
8.2 Repeatability <\/td>\n<\/tr>\n
14<\/td>\n8.3 Reproducibility <\/td>\n<\/tr>\n
15<\/td>\n9 Test report <\/td>\n<\/tr>\n
16<\/td>\nAnnex A (informative) Typical chromatograms
Figure A.1 \u2014 Typical chromatogram of baby food (pre-cooked flours of diastased maize and tapioca) spiked with 250,0 \u00b5g\/kg fumonisin B1 (FB1) and 62,5 \u00b5g\/kg fumonisin B2 (FB2)
Figure A.2 \u2014 Typical chromatogram of baby food (biscuits containing wheat, barley, rye, rice and maize) naturally contaminated with 164,8 \u00b5g\/kg FB1 and 35,6 \u00b5g\/kg FB2 <\/td>\n<\/tr>\n
17<\/td>\nAnnex B (informative) Precision data
Table B.1 \u2014 Precision and recovery data for fumonisins B1+B2 in baby food <\/td>\n<\/tr>\n
18<\/td>\nTable B.2 \u2014 Precision and recovery data for fumonisin B1 in baby food <\/td>\n<\/tr>\n
19<\/td>\nTable B.3 \u2014 Precision and recovery data for fumonisin B2 in baby food <\/td>\n<\/tr>\n
20<\/td>\nAnnex C (informative) Comparison between the method in this document and EN 14352:2004 and EN 13585:2001 on fumonisins in maize
Table C.1 \u2014 Comparison between the method in this document and EN 14352:2004 and EN 13585:2001 <\/td>\n<\/tr>\n
21<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection after pre-column derivatisation<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2015<\/td>\n24<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338164,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338156","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338156","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338164"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338156"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338156"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338156"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}