{"id":338091,"date":"2024-10-19T23:39:04","date_gmt":"2024-10-19T23:39:04","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-141642014\/"},"modified":"2024-10-25T22:44:56","modified_gmt":"2024-10-25T22:44:56","slug":"bs-en-141642014","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-141642014\/","title":{"rendered":"BS EN 14164:2014"},"content":{"rendered":"

This European Standard specifies a method for the determination of vitamin B6<\/sub> in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6<\/sub> is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The \u03b2-glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 [1] by which the different vitamins of vitamin B6<\/sub> (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard, EN 14166 [2], determines the total vitamin B6<\/sub> by microbiological assay.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
4<\/td>\nContents Page <\/td>\n<\/tr>\n
5<\/td>\nForeword <\/td>\n<\/tr>\n
6<\/td>\n1 Scope
2 Normative references
3 Principle
4 Reagents <\/td>\n<\/tr>\n
10<\/td>\n5 Apparatus
6 Procedure
6.1 Preparation of the test sample
6.2 Preparation of the sample test solution
6.2.1 Extraction <\/td>\n<\/tr>\n
11<\/td>\n6.2.2 Enzyme treatment and transformation steps
6.2.3 Identification
6.2.4 Determination by HPLC
7 Calculation <\/td>\n<\/tr>\n
12<\/td>\n8 Test report <\/td>\n<\/tr>\n
13<\/td>\nAnnex A (informative) Example of a chromatogram
Figure A.1 \u2014 Example for an HPLC quantification of pyridoxine in milk powder <\/td>\n<\/tr>\n
14<\/td>\nAnnex B (informative) Precision data
Table B.1 \u2014 Precision data for the determination of vitamin B6 <\/td>\n<\/tr>\n
15<\/td>\nTable B.2 \u2014 Precision data for the determination of vitamin B6 in reconstituted infant formula <\/td>\n<\/tr>\n
16<\/td>\nAnnex C (informative) Sample treatment option without acid hydrolysis <\/td>\n<\/tr>\n
17<\/td>\nAnnex D (informative) Examples for molar absorption coefficients
Table D.1 \u2014 Examples for molar absorption coefficients (\u03b5) of vitamin B6 compounds [6], [13] <\/td>\n<\/tr>\n
18<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Determination of vitamin B6 by high performance chromatography<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2014<\/td>\n20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338097,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338091","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338091","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338097"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338091"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338091"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338091"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}