{"id":338305,"date":"2024-10-19T23:40:15","date_gmt":"2024-10-19T23:40:15","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-ts-15634-52016\/"},"modified":"2024-10-25T22:46:19","modified_gmt":"2024-10-25T22:46:19","slug":"bsi-pd-cen-ts-15634-52016","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-ts-15634-52016\/","title":{"rendered":"BSI PD CEN\/TS 15634-5:2016"},"content":{"rendered":"

This Technical Specification specifies a procedure for the qualitative detection of species specific DNA from white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex real-time PCR based on the genes MADS-D (mustard) and lectin (soya) [1]. A mustard content of 10 mg\/kg or greater and a soya content of 10 mg\/kg or greater can be detected with a probability of > 95 %.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
4<\/td>\nContents Page <\/td>\n<\/tr>\n
5<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
6<\/td>\n1 Scope
2 Principle
3 Reagents <\/td>\n<\/tr>\n
7<\/td>\nTable 1 \u2014 Primers and probes for the real-time PCR <\/td>\n<\/tr>\n
8<\/td>\n4 Apparatus and equipment
5 Procedure
5.1 General
5.2 Sample preparation <\/td>\n<\/tr>\n
9<\/td>\n5.3 DNA extraction with CTAB
5.4 DNA purification by means of solid phase extraction <\/td>\n<\/tr>\n
10<\/td>\n5.5 Measuring the mass concentration of the extracted DNA and setting to target concentration
5.6 Real-time PCR
Table 2 \u2014 Reaction batch master mix soya, final volume\/concentration in the reaction vial <\/td>\n<\/tr>\n
11<\/td>\nTable 3 \u2014 Reaction batch master mix mustard, final volume\/concentration in the reaction vial
5.7 Temperature\/Time program
6 Validation status and performance criteria
6.1 General information on the interpretation of the real-time PCR <\/td>\n<\/tr>\n
12<\/td>\nTable 4 \u2014 Temperature\/Time program for plastic reaction vials
6.2 Reliability of the method
6.2.1 Setup of the interlaboratory study
6.2.2 Results of the interlaboratory study samples
6.2.3 Qualitative interpretation <\/td>\n<\/tr>\n
13<\/td>\nTable 5 \u2014 Interlaboratory study: Soya\/Mustard, all laboratories, qualitative interpretation (10 laboratories) <\/td>\n<\/tr>\n
14<\/td>\n7 Test report <\/td>\n<\/tr>\n
15<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Detection of food allergens by molecular biological methods – Mustard (Sinapis alba) and soya (Glycine max). Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2016<\/td>\n18<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338312,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[967,2641],"product_tag":[],"class_list":{"0":"post-338305","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-67-120-10","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338305","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338312"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338305"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338305"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338305"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}