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BS EN ISO 20150:2019

$102.76

Footwear and footwear components. Quantitative challenge test method to assess antifungal activity

Published By Publication Date Number of Pages
BSI 2019 22
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This document specifies quantitative challenge test methods for evaluating the antifungal activity of footwear and footwear components.

This document is applicable only to footwear and components that claim to have antifungal (antimycotic) properties or antimicrobial properties.

Two methods can be applied. The choice of method depends on the material properties and test microorganisms. Dynamic challenge test method can be applied to all types of materials. For single absorbent materials, static challenge test method is recommended. Brief descriptions of each method are given in 11.2 and 11.3.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
7 Foreword
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Safety
6 Apparatus
6.1 General
11 7 Reagents and culture medium
7.1 General
7.2 Water
7.3 Malt medium
7.3.1 Composition
7.3.2 Preparation
12 7.4 Malt extract agar (MEA) medium
7.4.1 Composition
7.4.2 Preparation
7.5 Physiological saline (sodium chloride solution)
7.5.1 Composition
7.5.2 Preparation
7.6 Wetting agent (nonionic surfactant)
7.7 Buffer solution
7.7.1 Buffer stock
13 7.7.2 Preparation of buffer stock
7.7.3 Preparation of buffer solution
8 Test microorganisms
9 Preparation of test inoculums
9.1 Indications for use of strains
14 9.2 Preparation of inoculums of Candida albicans
9.3 Preparation of test spore suspension of filamentous micro-fungi
10 Preparation of test specimens
10.1 General
15 10.2 Test specimen
10.3 Pre-treatment of test specimen
11 Test procedure
11.1 Summary of test methods
11.2 Dynamic challenge tests
11.2.1 Inoculation
16 11.2.2 Neutralization and elution after inoculation (time 0 h)
11.2.3 Incubation
11.2.4 Neutralization and elution after incubation (time 24 h)
11.2.5 Determination of the number of viable micro-fungi
11.3 Static challenge test
11.3.1 Inoculation
17 11.3.2 Neutralization and elution after inoculation (time 0 h)
11.3.3 Incubation
11.3.4 Neutralization and elution after incubation (time 24 h)
11.3.5 Determination of the number of viable micro-fungi
12 Expression of results
12.1 Calculation of the number of viable micro-fungi
12.2 Judgement of test effectiveness
18 12.3 Calculation of antifungal activity ratio
13 Test report
20 Bibliography
BS EN ISO 20150:2019
$102.76