BS EN ISO 20150:2019
$102.76
Footwear and footwear components. Quantitative challenge test method to assess antifungal activity
Published By | Publication Date | Number of Pages |
BSI | 2019 | 22 |
This document specifies quantitative challenge test methods for evaluating the antifungal activity of footwear and footwear components.
This document is applicable only to footwear and components that claim to have antifungal (antimycotic) properties or antimicrobial properties.
Two methods can be applied. The choice of method depends on the material properties and test microorganisms. Dynamic challenge test method can be applied to all types of materials. For single absorbent materials, static challenge test method is recommended. Brief descriptions of each method are given in 11.2 and 11.3.
PDF Catalog
PDF Pages | PDF Title |
---|---|
2 | National foreword |
4 | European foreword |
7 | Foreword |
9 | 1 Scope 2 Normative references 3 Terms and definitions |
10 | 4 Principle 5 Safety 6 Apparatus 6.1 General |
11 | 7 Reagents and culture medium 7.1 General 7.2 Water 7.3 Malt medium 7.3.1 Composition 7.3.2 Preparation |
12 | 7.4 Malt extract agar (MEA) medium 7.4.1 Composition 7.4.2 Preparation 7.5 Physiological saline (sodium chloride solution) 7.5.1 Composition 7.5.2 Preparation 7.6 Wetting agent (nonionic surfactant) 7.7 Buffer solution 7.7.1 Buffer stock |
13 | 7.7.2 Preparation of buffer stock 7.7.3 Preparation of buffer solution 8 Test microorganisms 9 Preparation of test inoculums 9.1 Indications for use of strains |
14 | 9.2 Preparation of inoculums of Candida albicans 9.3 Preparation of test spore suspension of filamentous micro-fungi 10 Preparation of test specimens 10.1 General |
15 | 10.2 Test specimen 10.3 Pre-treatment of test specimen 11 Test procedure 11.1 Summary of test methods 11.2 Dynamic challenge tests 11.2.1 Inoculation |
16 | 11.2.2 Neutralization and elution after inoculation (time 0 h) 11.2.3 Incubation 11.2.4 Neutralization and elution after incubation (time 24 h) 11.2.5 Determination of the number of viable micro-fungi 11.3 Static challenge test 11.3.1 Inoculation |
17 | 11.3.2 Neutralization and elution after inoculation (time 0 h) 11.3.3 Incubation 11.3.4 Neutralization and elution after incubation (time 24 h) 11.3.5 Determination of the number of viable micro-fungi 12 Expression of results 12.1 Calculation of the number of viable micro-fungi 12.2 Judgement of test effectiveness |
18 | 12.3 Calculation of antifungal activity ratio 13 Test report |
20 | Bibliography |