BS ISO 21322:2020

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Cosmetics. Microbiology. Testing of impregnated or coated wipes and masks

Published By	Publication Date	Number of Pages
BSI	2020	36

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Categories: 07.100.40 - Cosmetics microbiology, BSI

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Description

This document gives guidance for the enumeration and/or detection of microorganisms present in a cosmetic product that is impregnated or coated onto a substrate (i.e. wipes and masks) where sampling and microbiological influence of the manufactured product presents particular challenges in terms of microbiological sampling and testing.

The principle of this document can also be applied to test similar products (e.g. cushion, impregnated sponge, etc.) or applicators (e.g. brush, puff, sponge, etc.) with modification of the procedure as appropriate.

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PDF Pages	PDF Title
2	National foreword
7	Foreword
8	Introduction
9	1 Scope 2 Normative references 3 Terms and definitions
10	4 Principle 4.1 General information 4.2 Selection of the test sample
11	4.3 Selection of the method 4.4 Recovery of microorganisms from the test sample 4.5 Enumeration of aerobic mesophilic microorganisms 4.5.1 General 4.5.2 Plate count method overview 4.5.3 Membrane filtration method overview
12	4.6 Detection of specified microorganisms by enrichment method 5 Diluents, neutralizers and culture media 5.1 General 5.2 Diluents and neutralizers 5.3 Culture media 5.3.1 Media for enumeration and detection 5.3.2 Media for preparation of spores of Bacillus subtilis 6 Apparatus and glassware 7 Strains of microorganisms
13	8 Handling of cosmetic products and laboratory samples 9 Procedure 9.1 General recommendation 9.2 Selection and preparation of the test sample 9.2.1 Selection of the test sample 9.2.2 Preparation of the initial suspension
14	9.3 Recovery of microorganisms 9.3.1 General 9.3.2 Stomaching 9.3.3 Shaking/Stirring 9.4 Enumeration of aerobic mesophilic microorganisms 9.4.1 General 9.4.2 Pour plate method
15	9.4.3 Surface spread method 9.4.4 Membrane filtration method 9.4.5 Incubation 9.4.6 Counting of colonies
16	9.5 Detection of specified microorganisms by enrichment method 9.5.1 General 9.5.2 Test for specified microorganisms 10 Expression of results 10.1 Enumeration of aerobic mesophilic microorganisms
17	10.2 Detection of specified microorganisms 11 Suitability test 12 Test report
18	Annex A (normative) Guidance on methods for microbiological testing of impregnated or coated products — Wipes and masks
22	Annex B (informative) Expression and interpretation of results
29	Annex C (normative) Suitability test method
34	Bibliography

Additional information

Status	Withdrawn
Pages	36
Publication Date	2020-06-22
Withdrawn Date	2022-01-07
ISBN	978 0 580 94130 6
Standard Number	BS ISO 21322:2020
Title	Cosmetics. Microbiology. Testing of impregnated or coated wipes and masks
Identical National Standard Of	ISO 21322
Descriptors	Microorganisms, Enumeration, Products, Testing, Microbiology
Publisher	BSI
Committee	CW/217
ICS Codes	07.100.40 - Cosmetics microbiology

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BS ISO 21322:2020

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