{"id":338688,"date":"2024-10-19T23:42:14","date_gmt":"2024-10-19T23:42:14","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-iso-ts-20224-32020\/"},"modified":"2024-10-25T22:48:35","modified_gmt":"2024-10-25T22:48:35","slug":"bsi-pd-iso-ts-20224-32020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-iso-ts-20224-32020\/","title":{"rendered":"BSI PD ISO\/TS 20224-3:2020"},"content":{"rendered":"

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of porcine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of porcine material derived from pig (Sus scrofa domesticus<\/i>) and wild boar (Sus scrofa<\/i>).<\/p>\n

The target sequence is a partial fragment of the Sus scrofa<\/i> beta actin (ACTB) gene, partial cds. (i.e. GenBank accession number DQ452569.1)[<\/sup> 1<\/sup><\/span>]<\/sup>, which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ? 95 % replicability at this concentration (LOD95 %<\/sub>).<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4 Scientific basis
5 Reagents and materials
5.1 General
5.2 PCR reagents <\/td>\n<\/tr>\n
11<\/td>\n6 Apparatus
7 Procedure
7.1 Preparation of the test portion\/sample
7.2 Preparation of DNA extracts
7.3 PCR setup
7.3.1 Reaction mixes <\/td>\n<\/tr>\n
12<\/td>\n7.3.2 PCR controls
7.3.3 Real-time PCR thermocycler plate set-up
7.4 Temperature-time programme
8 Accept\/reject criteria
8.1 General <\/td>\n<\/tr>\n
13<\/td>\n8.2 Identification
9 Validation status and performance criteria
9.1 General
9.2 Robustness <\/td>\n<\/tr>\n
14<\/td>\n9.3 Reproducibility
9.4 Sensitivity <\/td>\n<\/tr>\n
17<\/td>\n9.5 Specificity <\/td>\n<\/tr>\n
19<\/td>\n10 Test report <\/td>\n<\/tr>\n
20<\/td>\nAnnex A (informative) BlastN 2.9.0 results for query of GenBank refseq_genomes (452 databases) <\/td>\n<\/tr>\n
25<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR – Porcine DNA detection method<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2020<\/td>\n26<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338696,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338688","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338688","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338696"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338688"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338688"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338688"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}